Figure 1.

Cross-blocking of human and mouse D8 Abs reveal a novel epitope. A, human antibody cross-blocking reveals two specificity groups (columns labeled 1 and 2). B, overlap between mouse and human mAbs identify a separate epitope for VACV-66. Black cells correspond to Abs that fully block D8 binding of each other and are thus assumed to share overlapping epitopes. Empty cells correspond to Abs that did not affect the D8 binding of each other. Relative strength of cross-blocking is indicated in shades of gray, because epitopes may overlap partially and allow simultaneous binding of both antibodies. Columns 1–4 indicate the four different specificity groups.