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. 2017 Nov 9;293(1):390–401. doi: 10.1074/jbc.M117.814541

Figure 3.

Figure 3.

Real-time binding kinetics using biolayer interferometry. mAbs were immobilized on anti-human Fc capture antibody sensors and the 1:1 binding of each Fab to monomeric D8 was measured by dipping into increasing concentrations (colored curves) of D8. Kinetic measurements were unaffected by antibody avidity. All antibodies bind with high affinity.