Figure 4.

Interference of D8 binding to CS-A or CS-E by human mAbs. Hexameric D8 was incubated with mAbs VACV-66, VACV-138, VACV-304, or alone prior to binding to GAG microarrays. Antibody blocking was assessed by comparing the amount of D8 and D8–mAb complex bound to the microarray. Blocking experiments were performed in triplicate (± S.E., error bars). Although VACV-66 does not block D8 binding to CS-A or CS-E, VACV-138 blocks binding to both. VACV-304 fully blocks binding to CS-A, whereas slight binding to CS-E was observed (∼25% of maximum binding).