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. 2017 Nov 20;293(1):148–162. doi: 10.1074/jbc.M117.813931

Figure 3.

Figure 3.

Perinatal deletion of Fhod3 results in juvenile lethality. A, PCR analyses of genomic DNAs from hearts of Fhod3flox/+;MCK-Cre+ and Fhod3flox/+;MCK-Cre− mice. The PCR product from the wild-type allele (Fhod3+) or the null allele (Fhod3−) was 329 bp in length (#3). The PCR product from the floxed allele (Fhod3flox) was 387 bp in length (#2). The 549-bp fragment was produced by Cre-mediated recombination (#1). Primers used are shown in Fig. 2A. B, survival curves of Fhod3 cKO (Fhod3flox/−;MCK-Cre+, n = 205) and control littermate (Fhod3flox/+;MCK-Cre+, n = 227; Fhod3flox/−;MCK-Cre−, n = 226; and Fhod3flox/+;MCK-Cre−, n = 245) mice. C, detection of the Fhod3 protein by immunoblot analysis. Proteins prepared from the heart of Fhod3 cKO (Fhod3flox/−;MCK-Cre+) and control littermate (Fhod3flox/+;MCK-Cre−) mice at the indicated embryonic or postnatal days were analyzed by immunoblot with the anti-Fhod3-C20. The loading amounts were verified by immunoblot with anti-α-tubulin or by fast green staining. D, representative images of Fhod3 cKO (Fhod3flox/−;MCK-Cre+) and control littermate (Fhod3flox/+;MCK-Cre−) mice at E17.5, P2, P6, and P10. The excised hearts are shown in right panels. Scale bars: left, 1 cm; right, 2 mm. E, body weight of Fhod3 cKO (Fhod3flox/−;MCK-Cre+) (n = 40) and control littermate (Fhod3flox/+;MCK-Cre−) (n = 34) mice from P0 to P15.