Figure 8.
Continuous infusion of phenylephrine induces hypertrophic changes in the Fhod3-deleted heart. A, survival curves after continuous infusion of phenylephrine (PE) for 2 weeks. TAM-treated Fhod3 iKO and TAM-treated control littermate mice were infused with PE (100 mg/kg/day) or saline for 2 weeks. B, representative images of hearts of TAM-treated Fhod3 iKO and TAM-treated control littermate mice after PE infusion. These experiments have been repeated three times with similar results. C, histological analyses of hearts of TAM-treated Fhod3 iKO (Fhod3flox/−;MerCreMer+, TAM(+)) and TAM-treated control littermate (Fhod3flox/−;MerCreMer−, TAM(+)) mice after PE infusion. Longitudinal sections of hearts were stained with hematoxylin and eosin. Scale bars, 3 mm. D, heart-to-body weight and kidney-to-body weight ratios after PE infusion were determined. †, p < 0.01; ‡, p < 0.001.