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. 2017 Nov 13;293(1):132–147. doi: 10.1074/jbc.M117.801944

Figure 3.

Figure 3.

Optn−/− cells show reduced recruitment of Atg12-5-16L1 complex to Wipi2-positive phagophores. A, representative images showing WT and KO MEFs, untreated or treated with EBSS for 2 h and stained with Wipi2 and Atg16L1 antibodies. Scale bar: 20 μm. B, graphs showing percentage (average ± S.D.) of Wipi2 puncta positive for Atg16L1 and percentage of Atg16L1 puncta positive for Wipi2. ***, p < 0.001; *, p < 0.05. n = 20 cells. C, graph showing average (± S.D.) area of Atg16L1 puncta in WT and KO MEFs, untreated or treated with EBSS for 2 h. ***, p < 0.001. D, representative images showing WT and KO MEFs, untreated or treated with EBSS for 2 h, stained with Wipi2 and Atg12 antibodies. Scale bar: 20 μm. E, graphs showing percentage (average ± S.D.) of Wipi2 puncta positive for Atg12 and Atg12 puncta positive for Wipi2. ***, p < 0.001; **, p < 0.01. n = 20 cells. F, graph showing average area of Atg12 puncta in WT and KO MEFs, untreated or treated with EBSS for 2 h. ***, p < 0.001; *, p < 0.05. Error bars: mean ± S.D.