Figure 7.

Optn forms a complex with Wipi2 and Atg5-12 conjugate. A, KO MEF cell lysates, expressing either GFP (Con), GFP-OPTN, or GFP-E478G were immunoprecipitated by GFP-Trap beads and subjected to Western blotting for indicated proteins. B, HEK 293 cell lysates, expressing either GFP alone (Con) or GFP-OPTN and subjected to immunoprecipitation by GFP-Trap beads, and immunoblotted for indicated proteins. C, optineurin tagged with GST or GST alone was immobilized on glutathione agarose beads and incubated with HEK cell lysates overexpressing HA-Atg5. Bound proteins were subjected to Western blotting for indicated proteins. D, optineurin tagged with GST or GST alone was immobilized on glutathione agarose beads and incubated with HEK cell lysates. Bound proteins were subjected to Western blotting for Wipi2 first and then Atg5.