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. 2017 Nov 13;293(1):132–147. doi: 10.1074/jbc.M117.801944

Figure 8.

Figure 8.

Ser-177 phosphorylation of optineurin promotes autophagosome formation, but it is not involved in recruitment to phagophore. A, KO MEFs transfected with either HA-OPTN or HA-S177A or HA-S177D were treated with EBSS for 2 h and stained for HA and LC3b. Scale bar: 20 μm. Graph shows average (± S.D.) number of LC3 dots per cell in KO MEFs alone (nonexpressing, NE) or KO MEFs expressing HA-OPTN or HA-S177A. n = 3 experiments, each experiment represents average of at least 20 expressing cells and nonexpressing cells. **, p < 0.001. B, KO MEFs transfected with either HA-OPTN or HA-S177D or HA-S177A were stained with HA and Atg12. Line scan indicates changes in intensity of HA tagged proteins along Atg12 puncta. Scale bar: 10 μm. C, confocal images of KO MEF expressing GFP-Optn and stained for pSer-177–Optn and Wipi2. Line scan indicates changes in intensity of pSer-177–Optn along Wipi2 puncta.