Content of pro-inflammatory markers in hippocampus and prefrontal cortex at 1, 15, and 30 days after CLP. TLR4, GFAP, and nNOS protein levels in hippocampus (A–C, respectively) and prefrontal cortex (D–F, respectively) were evaluated by Western blotting. Scattered individual data points (n = 6) and standard deviation are represented. Representative Western blots are demonstrated with graphs. Differences between sham and CLP groups in each day were considered significant when p < 0.05 according to Student's t test (two-tailed) analysis. Individual p values are depicted when differences were detected.