Figure 4.

RAGE ligands, sRAGE, and RAGE in serum and brain. The circulating content of the RAGE ligands S100B, CML, HSP70, and HMGB1 and of the sRAGE isoform in serum was assessed by ELISA (A). Values represent relative quantification considering control (sham group) as 100%. The immunocontent of CML, HMGB1, HSP70, and RAGE in hippocampus (B) and prefrontal cortex C) was assessed by Western blotting. Representative Western blots are demonstrated. Scattered individual data points (n = 6) and standard deviation are represented for all data. Differences between sham and CLP groups were considered significant when p < 0.05 according to Student's t test (two-tailed) analysis (*, p < 0.05, and **, p < 0.001).