Figure 3.

Expression of SCD-associated UBIAD1 (N102S) leads to enhanced synthesis of cholesterol in SV-589 cells. SV589(ΔUBIAD1)/pMyc-UBIAD1 (WT) and SV589(ΔUBIAD1)/pMyc-UBIAD1 (N102S) cells were set up on day 0 at a density of 4 × 10⁵ cells/60-mm dish in medium A containing 10% FCS and 1 mm mevalonate. On day 1, the cells were switched to medium A containing 10% FCS. On day 2, the cells received the identical medium supplemented with either 15 μCi/ml [¹⁴C]acetate (A) or 10 μCi/ml [³H]mevalonolactone (B) and incubated for 4 h at 37 °C. Incorporation of [¹⁴C]acetate and [³H]mevalonolactone into [³H]cholesterol was determined by TLC and scintillation counting as described in the legend to Fig. 2. Each value is the mean of triplicate incubations (± standard error). The p values were calculated using the Student's t test: NS, not significant; ***, p ≤ 0.001.