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. 2017 Nov 16;293(1):203–214. doi: 10.1074/jbc.M117.818930

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Figure 4. — Size-exclusion chromatography and CD spectroscopy analysis of mouse CLEC3A. A, full-length CLEC3A (CLEC3A-flc), CLEC3A-Ex3, or CLEC3A-Ex2 were analyzed on a size-exclusion column and the elution positions compared with molecular mass standards. Elution fractions were collected and analyzed by SDS-PAGE followed by immunoblot with the Affinity-purified antiserum against mouse CLEC3A-Ex3 in case of CLEC3A and CLEC3A-Ex3 and by silver staining in case of CLEC3A-Ex2. V₀, void volume; V_t, total volume. B, full-length CLEC3A (CLEC3A-flc), CLEC3A-Ex3, and CLEC3A-Ex2 were analyzed by CD spectroscopy. All spectra showed minima at 207 nm and 227 nm, characteristic for α-helical structure.