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Binding of CLEC3A to plasminogen. A, for ELISA-style binding assay, human CLEC3A was coated on 96-well plates at 1 μg/well followed by incubation with human plasminogen in serial dilutions. Error bars indicate ±S.D. B, for surface plasmon resonance analysis, human plasminogen was immobilized on a sensor chip and serial dilutions of human full-length CLEC3A (0.315–5 μm) were passed over the chip. Both methods showed a concentration-dependent binding of plasminogen to CLEC3A.
