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. 2017 Nov 16;293(1):100–111. doi: 10.1074/jbc.M117.815704

Figure 4.

Figure 4.

Residues from helices α1 and α2 are important for the function of MapZ in suppressing flagellar motor switching. A, bar graphs showing the percentage of time that P. aeruginosa PAO1 and overexpression mutant strains underwent clockwise (CW) rotation. The sample sizes are 32, 30, 30, 28, 27, 25, 27, and 31 cells for PAO1, mapZ+, mapZ(R13A)+, mapZ(D93A)+, mapZ(H97A)+, mapZ(R99A)+, mapZ(R100A)+, and mapZ(L104A)+, respectively. The corresponding videos for representative rotating cells can be found in Movies S1–S8. Data are representative of two independent experiments. B, protein expression levels of MapZ and MapZ mutants in P. aeruginosa by Western blotting using HA-specific antibody. RNAP-β served as a loading control and was visualized using RNAP-β–specific antibody. Blots are representative of two independent experiments.