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. 2017 Nov 15;293(1):345–358. doi: 10.1074/jbc.M117.816884

Figure 2.

Figure 2.

MC Munc13-4 is required for a full anaphylactic response. Mice were sensitized i.v. with anti-DNP IgE and challenged i.v. with DNP-HSA. Sham, non-sensitized but challenged B6 mice. A, change in body core temperature from baseline (ΔT). Circle, mean; error bar, S.E. C and D, ΔT 5 min (B) and 50 min (C) post-challenge. Line, mean; box, 25th–75th percentile; whiskers, 5th–95th percentile; n = number inside boxes, applies to A–C. D, plasma histamine levels 3 min post-challenge. Bar, mean; error bar, S.E.; n = number inside bars. E, representative lip sections 15 min post-challenge stained with toluidine blue to identify MC metachromatic granules. Scale bar = 40 μm. F, quantification of MC degranulation in lips and tongues based on proportion of granules outside the MC: low, <10%; moderate, 10–50%; and severe, >50%. Number of cells inside bars, number of animals in parentheses. # = p < 0.05, † = p < 0.01, * = p < 0.001; all comparisons are to Munc13-4+/+ unless otherwise indicated.