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. 2017 Nov 15;293(1):345–358. doi: 10.1074/jbc.M117.816884

Figure 4.

Figure 4.

Munc13-4 controls only regulated exocytosis in MCs. PCMCs sensitized with anti-DNP IgE and then challenged with DNP-HSA (DNP) or PMA/ionomycin (PI). Color legend in A applies to all panels. Regulated exocytosis was monitored by measuring secretion of histamine (A) or β-hexosaminidase (B and C) as fraction of total cell content 30 min post-stimulation. n = 4; circle and bar, mean; error bar, S.E. Constitutive exocytosis was monitored by measuring secretion of TNFα (D) and IL4 (E) 6 h post-stimulation. n = 8. Secretion independent of exocytosis was monitored by measuring PGD2 (F) and LTC4 (G) 30 min post-stimulation. n = 4. Line, mean; box, 25th–75th percentile; whiskers, 5th–95th percentile. * = p < 0.001; all comparisons are to Munc13-4+/+ unless otherwise indicated.