Figure 6.
Ectopic IRF5 alters the expression of genes associated with mitochondrial apoptosis. A, C-5B replicon cells were transiently transfected with empty vector (V) control or GFP-IRF5 (IRF5) plasmid, and 36 h later, total RNA was isolated for analysis of gene expression using the liver cancer RT2 ProfilerTM PCR array. Data from three independent replicates is shown in a clustergram (heat map) representing relative expression levels for all samples and all genes included on the real-time qPCR array. Red, up-regulated expression; green, down-regulated expression. B, same as A except data are represented as -fold change compared with the control group after normalization to housekeeping genes. Genes with >1.5-fold change in expression between empty vector control and IRF5-overexpressing cells are shown. C, representative Western blot of genes in the liver cancer RT2 ProfilerTM PCR array. Data are representative of three independent replicates performed in each cell line. D, summarized results from densitometry analysis of C. Data are from three independent replicates; plotted values are means ± S.D. (error bars) (*, p < 0.05; **, p < 0.001; ***, p < 0.0001).