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. 2017 Nov 2;292(52):21243–21252. doi: 10.1074/jbc.RA117.000123

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 5. — Adduction of alkynyl-analog 8 to cellular protein. A, RAW264.7 cells were treated with curcumin (75 μm) or alkynyl-analog 8 (1–75 μm). B, RAW264.7 cells were treated with alkynyl-analogs 8 (oxidizable) or 7 (non-oxidizable) at 10 and 75 μm, respectively. C, pretreatment of cells with curcumin, non-oxidizable 4, or oxidizable 11 (150 μm) followed by treatment with 8 (75 μm) or vehicle. Rhodamine-azide clicked protein samples (25 μg) were resolved by SDS-PAGE (4–20%) and analyzed by fluorescence or Coomassie stain.