EcR and βftz-F1 are required for Mmp expression.
A, fat body cell dissociation in EcRDN-overexpressing and βftz-F1 down-regulated animals at 12 h APF (left panel) and βftz-F1-overexpressing animals at 6 h APF (right panel). B, Mmps protein levels in the fat body of EcRDN-overexpressing and βftz-F1 RNAi animals at 12 h APF (left panel) and in the fat body of βftz-F1-overexpressing animals at 6 h APF (right panel). C and D, Mmps mRNA levels in the fat body of EcRDN-overexpressing and βftz-F1 down-regulated animals at 12 h APF (C) and in the fat body of βftz-F1-overexpressing animals at 6 h APF (D). E, the columns show developmental changes in the mRNA levels of βftz-F1 in fat body, and the curve shows the degree of fat body dissociation of w1118 animals at 3-h intervals. βftz-F1 mRNA levels peak at 9 h APF. F, Mmps mRNA levels in EGFP-overexpressing (as a control) and βftz-F1-overexpressing Kc cells.