Figure 4.

E75, DHR3, and Blimp-1 regulate the expression of Mmps and βftz-F1. A, fat body cell dissociation in animals in which E75A was overexpressed, DHR3 expression was reduced by RNAi, combined E75A overexpression and DHR3 RNAi, and Blimp was overexpressed at 12 h APF (left panel). Fat body cell dissociation in animals in which DHR3 was overexpressed, E75 expression was reduced by RNAi, combined DHR3 overexpression and E75 RNAi, and Blimp expression was reduced by RNAi at 6 h APF (right panel). B, βftz-F1 and Mmp expression in the fat body of E75A overexpression (left panel), DHR3 RNAi (middle panel), and combined E75A overexpression and DHR3 RNAi (right panel) animals. C, βftz-F1 and Mmp expression in the fat body of E75 RNAi (left panel), DHR3 overexpression (middle panel), and combined E75-RNAi and DHR3 overexpression animals (right panel). D, βftz-F1 and Mmp expression levels in the Blimp-1 RNAi fat body (left panel); βftz-F1 and Mmp expression levels in the Blimp-1 overexpressing fat body (middle panel) and Kc cells (right panel). E, the columns show developmental changes in mRNA levels of E75 (left panel), Blimp-1 (middle panel), and DHR3 (right panel) in the fat body, and the curve shows the degree of fat body dissociation in w¹¹¹⁸ animals at 3-h intervals.