Figure 3.

Effects of IRF-5 on IAV-induced inflammatory cytokine expression. A and B, PBMCs were transfected with the indicated expression constructs or empty vector for 24 h, infected with the WSN virus (MOI = 1) or mock-infected for 24 h, and subjected to real-time RT-PCR (A) and ELISA (B). C, THP-1 cells were transfected with the indicated IRF-5 siRNAs or siRNA control for 48 h before real-time RT-PCR (left) and Western blot (right) analyses. D and E, experiments were performed similar to those described in A and B, except that IRF-5 siRNAs or siRNA control were used. All experiments were repeated at least three times with consistent results. Bar graphs present means ± S.D., n = 3 (**, p < 0.01; *, p < 0.05; n.s., not significant).