AngII induces SRC-2 and VEGF expression.
A, immunoblotting for SRC-2 in H9c2 cells treated with AngII for the indicated times. GAPDH and total protein are used as loading controls. qPCR for SRC-2 was performed in H9c2 cells treated with AngII for 24 h. Rpl32 was used as in internal control, and data are presented relative to vehicle. B, qPCR for VEGF from isolated adult cardiomyocytes from mice treated with vehicle or tamoxifen to induce knockout of SRC-2. After isolation, cardiomyocytes were treated with vehicle or AngII for 12 h. Rpl32 was used as an internal control, and data are presented relative to vehicle control. Data are presented relative to vehicle control. *, p ≤ 0.05; ***, p ≤ 0.001. Error bars, S.E.