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. 2018 Jan 12;49:5. doi: 10.1186/s13567-017-0500-z

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Morphometric analysis of BREs. The thickness of the unchallenged tracheal epithelium (A) was significantly reduced at 48 (*p ≤ 0.001) and 72 (**p = 0.01) hpc when compared with T0. However, in absolute terms, such differences were extremely small and always less than 5 µm. No significant difference was observed between non-infected and challenged tracheal explants (p ≥ 0.05). The thickness of the unchallenged bronchial epithelium (B) was not significantly modified along the entire time course of the experiment. No significant difference was observed between non-infected and challenged bronchial explants (p ≥ 0.05). The surface of the unchallenged alveolar walls—expressed as percentage of the entire field of observation—was not significantly modified along the entire the time course of the experiment (C). No significant difference was observed between non-infected and challenged lung explants (p ≥ 0.05). Data were represented as the mean ± SD.