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. 2018 Jan 12;49:5. doi: 10.1186/s13567-017-0500-z

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Immunohistochemistry for Mmm on challenged BREs. Mmm-IR was detectable within the MALT, the IHC pattern resembling that of the dendritic-cells (A; tracheal explant, T24). The presence of Mmm was observed upon the bronchiolar epithelium (B; lung explant, T1), along the endothelial surface of large blood vessel (C; tracheal explant, T24) and inside the cytoplasm of alveolar macrophages (D; lung explant, T1). At higher magnification, the Mmm-IR was apparently seen inside the cytoplasm of bronchiolar epithelial cells (E; lung explant, T1) and of endothelial cells (F; lung explant, T1). Mayer’s hematoxylin counterstain. Scale bar: 10 µm (E), 20 µm (D, F), 50 µm (A), 100 µm (B, C).