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. 2018 Jan 12;49:5. doi: 10.1186/s13567-017-0500-z

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Immunohistochemistry for Mmm after pre-incubation with bovine sera. The pre-incubation of the pathogen with CBPP-negative serum did not modify the IHC pattern of Mmm, whose antigens were easily detected upon the alveolar surface (A). In contrast, Mmm antigens were undetectable in lung explant after the pre-incubation with a CBPP-positive serum (B). Mayer’s hematoxylin counterstain. Scale bar: 50 µm.