Fig. 3.

Overexpression of miR-200a induced chemoresistance was mediated through TP53INP1 and YAP1. a The Cytoscape map shows the intersection between the miR-200a predicted targets and the p53/p73 interacting proteins. b Sequence alignment of human miR-200a within the 3’-UTRs of TP53INP1. The seed sequence of miR-200a matches the 3’-UTRs of TP53INP1. Mutations within the 3’-UTRs of TP53INP1 in the mutant luciferase reporter constructs are as shown. The psiCHECK2-TP53INP1 vector was used for the luciferase assays. c 293 T cells were transfected with the indicated plasmids and oligonucleotide. Firefly luciferase activity was normalized with Renilla luciferase activity. Relative luciferase activities are presented. Data represent three independent experiments in triplicate. d The expression of TP53INP1, YAP1 and p73 were evaluated using western blotting on samples from pMR-miR-200a and pMR-miR-Ctrl transfected MDA-MB-231 cells. GAPDH was used as a control. Downstream effectors of p73, including BAX, PUMA, and Bim, were also detected by western blotting. The data were derived from three replicated experiments. *p < 0.05