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. 2018 Jan 12;15:12. doi: 10.1186/s12974-017-1021-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Spinally delivered lentiviral vector LV-shIL-33 inhibits the expression of IL-33 in the dorsal spinal cord. a Representative real-time PCR of IL-33 mRNA expression in the rat ipsilateral spinal dorsal horns tissue. The fold change of IL-33 levels in the sham group was set as 1.0 for the quantifications (n = 6 in each group). b Representative Western blots of the IL-33 protein expression in the rat ipsilateral spinal dorsal horn tissue. β-tubulin was used as a loading control. The results are expressed as the ratio of IL-33 to β-tubulin, and the control was set at 1.0 (n = 6 in each group). c Quantification of immunofluorescence signals for IL-33 in the rat ipsilateral spinal dorsal horns (n = 4–5 in each group). d Representative immunofluorescence detection of IL-33 in the rat ipsilateral spinal dorsal horns. Panels f–j are higher-power magnifications of the boxed areas in the panels a–e. Bars = 50 μm (n = 6 in each group). The values are the mean ± SEM (^##p < 0.01 versus sham; **p < 0.01 versus the LV-NC group or the vehicle group)