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. 2018 Jan 12;8:2. doi: 10.1186/s13395-017-0148-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — RNA interference against DUX4c suppresses the disorganized myotube phenotype. dFSHD primary myoblasts were transfected with the indicated siRNAs and differentiated. a At day 8, microscope pictures were obtained under white light. b The cells were then fixed, and troponin T (green) was detected by immunofluorescence. Nuclei were stained with DAPI. Scale bar: 30 μm. c The average (and SD) numbers of nuclei per cluster and clusters per field were counted (10 fields per culture). The proportion of nuclei per cluster is shown in grayscale. ***p < 0.001 was considered significant