Table 1.
Performance characteristics of each MSI marker for the identification of MMR-deficient CRCs in the test set (317 CRCs)
| Marker | References | Sensitivity% (95% CI) | Specificity% (95% CI) for pMMR | PPV% (95% CI) for dMMR | NPV% (95% CI) for pMMR | |
|---|---|---|---|---|---|---|
| CAT25 | QMVR (106–108 bp) |
for dMMR (n = 105) | 94.3 (88.1–97.4) | 100.0 (98.2–100) | 100 (96.3–100) | 97.3 (94.1–98.7) |
| dMLH1 (n = 45) | 93.3 (82.1–97.7) | |||||
| dMSH2 (n = 45) | 100 (92.1–100) | |||||
| dMSH6 (n = 15) | 80.0 (54.8–93.0) | |||||
| NR21 | QMVR (131–134 bp) |
for dMMR (n = 105) | 91.4 (84.5–95.4) | 99.5 (97.4–99.9) | 99.0 (94.3–99.8) | 95.9 (92.4–97.8) |
| dMLH1 (n = 45) | 95.6 (85.2–98.8) | |||||
| dMSH2 (n = 45) | 97.8 (88.4–99.6) | |||||
| dMSH6 (n = 15) | 60.0 (35.7–80.2) | |||||
| NR27 | QMVR (153–156 bp) |
for dMMR (n = 105) | 94.3 (88.1–97.4) | 97.6 (94.6–99.0) | 95.2 (89.2–97.9) | 97.2 (94.0–98.7) |
| dMLH1 (n = 45) | 95.6 (85.2–98.8) | |||||
| dMSH2 (n = 45) | 100 (92.1–100) | |||||
| dMSH6 (n = 15) | 73.3 (48.0–89.1) | |||||
| BAT26 | QMVR (175–177 bp) |
for dMMR (n = 105) | 95.2 (89.3–97.9) | 98.1 (95.2–99.3) | 96.2 (90.5–98.5) | 97.7 (94.6–99.0) |
| dMLH1 (n = 45) | 97.8 (88.4–99.6) | |||||
| dMSH2 (n = 45) | 100 (92.1–100) | |||||
| dMSH6 (n = 15) | 73.3 (48.0–89.1) | |||||
PPV positive predictive value, NPV negative predictive value