(A) Typical examples of sagittal diagrams showing orx/hcrt cells found at around 0.84 mm from the midline. Open circles denote orx/hcrt-eGFP cells without tracer and filled circles are orx/hcrt-eGFP cells which contained tracer after injections into LC or VTA (as labelled above the sketches). Similar maps were created for each parasagittal section in the lateral range 0.48–1.08 mm (Paxinos & Franklin, 2001, figs 105–110). (B) Proportion of orx/hcrt-eGFP cells that contained tracer after it was injected into the LC. The cells were grouped into four distinct hypothalamic regions. No statistically significant differences between regions were found (P > 0.05, Fisher’s exact test). Figures inside bars are number of eGFP cells with tracer/total number of eGFP cells. (C) Proportion of orx/hcrt-eGFP cells that contained tracer after it was injected into the VTA, grouped into four hypothalamic regions. No statistically significant differences between regions were found (P > 0.05, Fisher’s exact test). The number of orx/hcrt cells projecting to LC versus VTA in the corresponding regions shown in B and C were also not significantly different (P > 0.05 for each region compared, Fisher’s exact test). Numbers inside bars are eGFP cells with tracer/total number of eGFP cells. ZI, zona incerta; PeF, perifornical area; DM/AH, dorsomedial/anterior hypothalamus; LH, lateral hypothalamus.