Figure 4. Cylinder locomotor activity test.

The number of hindlimb steps in the cylinder (A) between genotypes and (B) across data collection timepoints (corresponding to age of the animals; 2–6 months; main effect of time). The number of forelimb steps (C) between genotypes and (D) across timepoints. The number of rears + lands; main effect between (E) genotypes and main effect (F) across timepoints. X-axis is testing the genotype or timepoint/animal age (mo); Y-axis is number of steps. The boundary of the box closest to zero indicates the 25^th percentile. The line within the box marks the median and the boundary of the box farthest from zero indicates the 75^th percentile. Whiskers (error bars) above and below the box indicate the 90^th and 10^th percentiles. Outlying points are indicated by open circles. Asterisks represents statistical significance between genotypes (* p<0.05; ** p<0.01, ***p<0.001). Bars indicate significance between timepoints with asterisks showing levels of significance (* p<0.05; ** p<0.01, ***p<0.001).