Figure 2.

Effects of anti-activator gene deletion on P_lasB-gfp expression kinetics. (A) Growth of strains in CAA medium. (B) P_lasB-gfp expression levels vs. time. Expression levels are normalized to OD₆₀₀. Inset has reduced x- and y-axes to emphasize expression timing. (C) P_lasB-gfp expression rates vs. time, showing time derivatives of OD-normalized expression levels. Inset has a reduced y-axis to emphasize expression rate peaks in the wild-type. (D) OD-normalized P_lasB-gfp expression levels vs. cell density. Inset has a reduced y-axis to emphasize the wild-type expression profile. In all panels, values represent means of three biological replicates. Error bars indicate s.e.m. (n = 3).