Figure 9.

Broader context and functional relationship between quorum and anti-activator regulons. (A) Comparison of an extended QS regulon with genes induced in the qteE qslA anti-activator mutant in early stationary phase. The extended QS regulon contains all QS-induced genes identified in the current study and in previously published QS studies (Hentzer et al., 2003; Schuster et al., 2003; Wagner et al., 2003). Venn diagram is not scaled to gene number. (B) Functional classification of induced genes. Functional classes and annotations were retrieved from the Pseudomonas Genome Database. Bars represent percent of each functional class represented in induced gene lists, scaled to the size of the wild-type percentage of all genes (1.4%). Induced gene lists for each sample were assembled from differentially expressed (DE) genes in early stationary phase as determined in DESeq2 using three biological replicates (false discovery rate α = 0.05, n = 3).