Figure 6.

Inhibitors of apoptosis proteins regulate wound healing efficiency of primary macrophages. (A) HeLa cells at 2.5 × 10⁴ per well in 24-well plates cultured in complete RPMI medium for 24 h at 37°C to form a cell monolayer before the wound was made by a scratch. HeLa cells were allowed to migrate for 24 h upon treating with and without BP and vascular endothelial growth factor, respectively. Images of the wound areas were captured using an Inverted Fluorescence Microscope, at 10× and compared with images taken immediately after scratch was made. (B) Wound area was quantified by analyzing the images using ImageJ, and the percentage closure of the wound area was plotted as mean ± SE. (C) HeLa cells described in (A) were cultured in media conditioned by primary macrophages stimulated with lipopolysaccharide (LPS) (CM1) and/or in media conditioned by primary macrophages stimulated with LPS and treated with birinapant (CM2). Images of the wound areas were captured using an Inverted Fluorescence Microscope, at 10× and compared with images taken immediately after scratch was made. (D) Wound area was quantified by analyzing the images using ImageJ, and the percentage closure of the wound area was plotted as mean ± SE. Images presented are the representative of three independent experiments done in triplicates. Statistical analysis was conducted using two-way ANOVA followed by the Bonferroni post-test (*p < 0.05 and **p < 0.01).