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Southern blot analysis of two transformed cell lines. Total DNA (10 μg) was digested with SacI. Transformed T3 cell lines TwFPS1 had three fragments and TwFPS2 had one fragments, while no hybridization signal was detected in WT cell suspensions. Lane C: part of hygromycin gene fragment, Lane 1: TwFPS1 T3 transgenic cell lines, Lane 2: TwFPS2 T3 transgenic cell lines, Lane 3: WT cell suspensions.
