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. 2018 Jan 9;8:2669. doi: 10.3389/fmicb.2017.02669

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Copyright © 2018 Peredo and Simmons.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Composite 3-D image (4 × 4 tiles, approx. 340 μm) showing Sphingomonas, Methylobacterium and Pseudomonas on the leaf surface of in vitro grown Arabidopsis. (A) Hybridization mix included probes EUB338 I, II and III (conjugated with Rhodamine, false colored in red), Methylobacterium mybm-1388 (Alexa-488pink) and Pseudomonas PSE227 (Alexa-647, yellow). (B) Detail showing cells in stomata area, box approximate 50 μm. (C) Image after background removal, box ~100 μm. (D) Result of automatic particle counting, indicating the suitability of images for automatic cell quantification (Fiji). Yellow bar, 50 μm.