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. 2018 Jan 9;8:2669. doi: 10.3389/fmicb.2017.02669

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Copyright © 2018 Peredo and Simmons.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Preferential microhabitat of two Methylobacterium species in monoisolate seed inculated in vitro grown Arabidopsis (100 X lens, Zeiss LSM780). (A–C) M. extorquens PA1 was preferentially found in the stomatic chambers. (A) FISH EUB338 I, II and III conjugated with Alexa-488; (B) Ortho projection of Z-stack images, 8 μm depth, FISH EUB338 I, II, and III conjugated with rhodamine. (C) Maximum intensity projection, composed of four images (4 μm depth). (D–F) M. adhaesivum B5A preferentially inhabits leaf surface. (D) FISH EUB338 I, II, and III conjugated with Alexa-488; (E) Ortho projection of Z-stack images, 9 μm depth, FISH EUB338 I, II, and III conjugated with rhodamine and mybm-1388 conjugated with Alexa-488. (F) Maximum intensity projection, composed of five images (5 μm depth). M. adhaesivum B5A on plant trichome. To facilitate visualization, in each plate plant fluorescence was false colored in cyan and bacteria were false colored in pink. Yellow bar, 10 μm.