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. 2018 Jan 9;11:731. doi: 10.3389/fnins.2017.00731

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Copyright © 2018 Waller, Wyles, Heath, Kazoka, Wollff, Shaw and Kirby.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quality control data analysis across CSF small RNA sequenced samples. Each sample was ran in duplicate across two lanes of a sequencing flow cell. Chimira was used to provide an overall assessment of the miRNA expression content in each sequenced sample across the two lanes. (A) Eight samples showed read counts that were considerably higher than the remaining 82 samples (^* outlier samples), these outlier samples would skew any differential expression analysis. (B) Upon removal of the 8 outlier samples each sample read count is now comparable for differential expression analysis.