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(A) Cartoon representation of a 5-SL form of the HIV-1 RRE with basepairing in the central junction. Stem, stem loop and central junction designations are indicated. (B) SAXS, SHAPE and aiSHAPE analysis suggests that the distal segment of stem I (S-I) folds back on itself to provide a cryptic Rev binding site, pre-organizing the RRE for optimal Rev multimerization. Three-dimensional RRE and Rev6-RRE complex model structures are shown. Adapted from Bai et al. (2014).
