Figure 5.

Dissecting alternative topologies for the HIV-1 RRE by native gel electrophoresis and in-gel SHAPE. The central panel illustrates that the RRE exists as an equilibrium mixture of two conformers that can be separated by prolonged electrophoresis. In-gel SHAPE indicates that the faster and slower migrating forms assume the 4-SL and 5-SL conformations, respectively. Adapted from Sherpa et al. (2015).