Figure 7.

Effects of platycodin D (PLD) on LXR transcriptional activity and LXRα, ABCG1 expression. Cells were transfected with LXRE-driven luciferase reporter vector (LXRE-tk-Luc) and β-galactosidase control vector (Promega). Six hours later, cells were treated with PLD for 12 h. Relative luciferase activity was determined by normalization with β-galactosidase activity (*p < 0.05, **p < 0.01). Effect of PLD on LXRα and ABCG1 expression. Cells were treated with PLD (5, 10, 20 µM) for 12 h. Protein samples were analyzed by western blot with specific antibodies. β-Actin was used as a control. The values presented are the means ± SD of three independent experiments and differences between mean values were assessed by one-way ANOVA with Tukey’s multiple comparison test (*p < 0.05, **p < 0.01).