Figure 8.

Knockdown of LXRα abrogated the effects of platycodin D (PLD) on lipid rafts cholesterol levels, and LPS induces inflammatory response in primary rat microglia cells. Cells were transfected with a siRNA specific for LXRα, or a scrambledsiRNA (negative control) as indicated. Then, the cells were treated with PLD (20 µM) for 12 h. The effect of siRNA on LXRα expression was detected by western blotting. Lipid raft cholesterol levels were detected. Meanwhile, the cells were treated with PLD (20 µM) for 12 h and stimulated by LPS for 24 h. Levels of TNF-α, IL-1β, and IL-6 in culture supernatants were measured by ELISA. The data presented are the means ± SD of three independent experiments and differences between mean values were assessed by one-way ANOVA with Tukey’s multiple comparison test (^#p < 0.05 vs. control group; *p < 0.05, **p < 0.01 vs. LPS group).