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. Author manuscript; available in PMC: 2018 Jan 14.
Published in final edited form as: Mol Carcinog. 2017 Apr 13;56(8):1935–1944. doi: 10.1002/mc.22651

Figure 2. The homozygosity of X. hellerii alleles defines the R(Diff) region.

Figure 2

The region that only contained homozygous gene on LG5 defined the R(Diff) region. For each fish sample, heterozygous and homozygous genes were plotted on LG5 of the X. hellerii genome chromosome assembly. The genotype of each fish is represented by two LG5 genotype plots. The left one shows heterozygous genes and the right one shows homozygous genes. Genotyping information from the BC1 hybrid defined the R(Diff) region to be 5.81Mbp long (between the two dashed lines) and consists of 164 gene models. 114 genes were genotyped in our RNA-Seq experiments and were homozygous for the X. hellerii allele. Genotyping coding genes within the R(Diff) region of BC5 hybrids showed 79 genes can be genotyped. 10 of which are heterozygous and 69 of which were homozygous. Known RAD-Tag markers that were mapped to the R(Diff) region are labeled on the side of the genotype plots. Fish 9 of BC1, and Fish 13 of BC5 were excluded from this figure (See Materials and Methods for detail).