Table 1.

Primer sequences, repeated motifs, fluorescent labeling and annealing temperatures of 21 microsatellite loci

Locus	Primer sequence (5′ - 3′)	Repeat motif	Size (bp)	Label	Annealing Temp. (°C)
5A26216	F: AAGCTTCAGAAGGTGGCACT R: CGTGCTGAGGGATTAAGGAA	(AC)18	104–122	FAM	58
5A26261	F: CTCATATGGGGCCAACAAAC R: CCATCATGAAAGGGTGCTCT	(GT)11	146–157	FAM	58
5A5305-1	F: GGGGAGGAAGTATAGGTGTGC R: AATCACACCTCTCCCACAGG	(TTTTTG)9	194–238	FAM	58
5A5111	F: GAGAAGAACGGTGGATGAGC R: CCAGTGCTCTTTCCTTTTGC	(CA)9	246–267	FAM	58
5A26254	F: TGTGCCAATACCTCACTCCA R: CATCCTCCCACCAGCATTAT	(TTTC)8(CT)23	134–174	VIC	58
5A266113	F: CATGGCAACCAAAACCACTT R: GGCTTTGCTGTGCTACCTGT	(AC)11	190–213	VIC	58
5A265175	F: CGAATGTGACCAAAAGAACAG R: CATGATTTGAGGGTCACCAA	(CA)16	217–240	VIC	58
5A5279	F: ATGAACCTGAATGGGAAGGA R: ATGCTGATGCCAGTGTTCTG	(AAAAG)8(AAGAG)11(AAAAGAG)7	252–369	VIC	58
5A5397	F: TCAGAATACTGATTGCATTTGTCC R: TGCTGCATAAAGACACGGAG	(ATTT)12	120–158	PET	58
5A265141	F: GTGGCATGCTTCACTCAGAA R: AAGCATGCAAAACAGACCAA	(CT)6(GT)12	173–199	PET	58
5A26681	F: TAGGTGCTCCAATGAACTCAA R: TGAGCTTGTTTGCTTGTTGG	(GAGAA)37	218–367	PET	58
5A265151	F: TGTATTTGGGGCAAATGTGA R: TCCGGTCTGTAAAGTCAGACAA	(AG)10	157–180	NED	58
5A265164	F: TGCTGAAATGGTCAACATCC R: TCTTCTATCTCCTCCCCCTGA	(TA)9	213–229	NED	58
5A26648	F: GGATTTATCTGTGGGATTTATCTGA R: GTTGGTTTGGATTTGCTGCT	(GAGAA)20	245–376	NED	58
Ans13	F: GCTAAGGTTGTTGCATTGATC R: TAGAGGCACAGACAAAGCAGA	(CA)9	127–161	FAM	53
Ans17	F: ACAAATAACTGGTTCTAAGCAC R: AGAGGACTTCTATTCATAAATA	(TA)3 (CA)6C(CA)3(TA)3	111–140	FAM	53
Ans21	F: TTCAGCATAAGTTCAGGCATG R: TATGGGTTAGTGTTCTTCTCA	(AT)2(GT)5(AT)2(GT)6	173–189	FAM	53
Aph19b	F: ATGGAGCAAGCAATCGTCTG R: AGCGTGAGGGTCTGCAGA	(CA)8	229–240	FAM	53
Aalμ1b	F: CATGCGTGTTTAAGGGGTAT R: TAAGACTTGCGTGAGGAATAG	(GT)15	66–90	VIC	53
Ans02	F: TTCTGTGCAGGGGCGAGTT R: AGGGAACCGATCACGACATG	(AG)17	196–232	VIC	53
Ans25	F: CACTTATTAATGGCACTTGAAA R: GTTCTCTTGTCACAACTGGA	(GT)18	251–269	VIC	53

FAM, 6-fluorescein amidite.