Skip to main content
. 2017 Aug 16;31(2):301–308. doi: 10.5713/ajas.17.0319

Table 3.

Primer sequences used in quantitative polymerase chain reaction

Genes Accession no. Product size (bp) Primer sequence
MyHC-I NM_213855 152 GCTGGACTACAACATCATAGGC
CTTTGCCCTTCTCAACAGGT
MyHC-IIa NM_214136 134 AAACCTCACGGAAGAGATGG
TCAGGGTGTTGACTTTGTCCT
MyHC-IIb NM_001123141 148 GATGTTCCTGTGGATGGTCA
CTCGTTGGTGAAGTTGATGC
MyHC-IIx NM_ 001104951 153 GAAACCGTCAAGGGTCTACG
CGCTTCCTCAGCTTGTCTCT
GAPDH NM_001034034.2 116 TCCAGCCTTCCTTCCTGGGCAT
GGACAGCACCGTGTTGGCGTAGA

MyHC, myosin heavy-chain; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.