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. 2018 Jan 10;8:986. doi: 10.3389/fphar.2017.00986

FIGURE 4.

FIGURE 4

Effect of adenine nucleotides on expression of CD25 and proliferation of BrdU-labeled cells. Lymphocytes were pre-incubated for 30 min with 250 μM ATP, ADP, AMP, or adenosine before ConA treatment or post-incubated with ATP at 30 min after ConA treatment. (A) Cells were incubated for 24 h with ConA. The lymphocytes were stained with PE-conjugated anti-CD25 mAb and FITC-conjugated anti-CD4 mAb. The percentage of CD4+ CD25+ cells was analyzed by flow cytometry. (B) Cells were incubated for 21 h with ConA. Lymphocyte proliferation was determined by BrdU cell proliferation assay. Each value represents the mean ± SE (n = 4). Significant difference between the vehicle control group and the ConA-treated group in the absence of ATP or other ligand is indicated by ###P < 0.001. Significant differences between ConA-treated groups without ligand treatment and the corresponding groups given the indicated ligand treatment are indicated by ∗∗∗P < 0.001 and ∗∗P < 0.01. (A) Represents three independent experiments and (B) represents two independent experiments.