Table 1.

Composition of tris extender for control and treatments

Components	Tris extender
	Control	T1	T2	T3
Tris buffer	73 ml	70.5 ml	68 ml	66 ml
Fructose	1.25 g	-	-	-
Egg yolk	20 ml	20 ml	20 ml	20 ml
Glycerol	7 ml	7 ml	7 ml	7 ml
Penicillin G sodium	80000 IU	-	-	-
Streptomycin	100 mg	-	-	-
Honey	-	2.5 ml	5 ml	7 ml