Table 1.
Rat primers and sequences for real‐time RT‐PCR
| Primers | ||
|---|---|---|
| Gene | Forward 5′–3′ | Reverse 5′–3′ |
| Actb | GACGGAATTTACGGCTCAACAT | AATTAGGAAAGTTGAGCCAATAATTACG |
| Bcl‐2 | GAGGGGCTACGAGTGGGATA | CGGTAGCGACGAGAGAAGTC |
| Bax | GCTGCAGAGGATGATTGCTGA | TAGAAAAGGGCAACCACCCG |
| Foxo1 | AAGAGTTAGTGAGCAGGCAAC | CAGAGCACAGGCAGTACACA |
| IGF‐1 | CAGTTCGTGTGTGGACCAAG | TCAGCGGAGCACAGTACATC |
| Gata4 | CTCTTCGTCCTTCGCTGGAG | GACTGGTCTCGAACACCCTG |
| Myh6 | AAGAGTGACAGGATGACGGA | GTCACCGTCTTGCCGTTTTC |
| Nkx2.5 | CCTCGGATTTCACACCCACA | CCGAGGCATCAGGTTAGGTC |
| Nppa | GAAGCGGGGGCGGCACTTAG | TTCGGTACCGGAAGCTGTTGCA |
| Nppb | AACAATCCACGATGCAGAAGC | GGCGCTGTCTTGAGACCTAA |
| Rhoa | GGTTTATGTGCCCACGGTGT | TACCGGCTCCTGCTTCATTTT |
| r18s | GCATGGCCGTTCTTAGTTGG | TGCCAGAGTCTCGTTCGTTA |
| miRNA analysis | |
|---|---|
| Assay name | Sequence |
| U6 snRNA | GTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACAGAGAAGATTAGCATGGCCCCTGCGCAAGGATGACACGCAAATTCGTGAAGCGTTCCATATTTT |
| snoRNA234 | CTTTTGGAACTGAATCTAAGTGATTTAACAAAAATTCGTCACTACCACTGAGA |
| miR‐1 | UGGAAUGUAAAGAAGUAUGUAU |
| miR‐30e | UGUAAACAUCCUUGACUGGAAG |
| miR‐99b | CACCCGUAGAACCGACCUUGCG |
| miR‐125b | ACAAGUCAGGCUCUUGGGACCU |
| miR‐133b | UUUGGUCCCCUUCAACCAGCUA |
| miR‐204 | UUCCCUUUGUCAUCCUAUGCCU |
| miR‐208b | AAGCUUUUUGCUCGCGUUAU |
| miR‐222 | CUCAGUAGCCAGUGUAGAUCCU |
This table presents the genes and miRNAs of interest with probe sequences and assay numbers. Actb, beta‐actin; Foxo1, Forkhead Box O1; Gata4, GATA transcription factor 4; Myh6, myosin heavy chain 6; Nkx2.5, Nk2 homeobox 5; Nppa, natriuretic peptide A; Nppb, brain natriuretic peptide; r18s, ribosomal 18 s; Rhoa, Ras homolog gene family, member A; Bcl2, Bcl2‐associated X protein; Bax, B‐cell lymphoma 2; IGF‐1, insulin‐like growth factor 1.