Figure 6.

Three‐dimensional imaging of LecA‐dependent staining of P. aeruginosa PAO1 biofilms with 17. A) P. aeruginosa PAO1 wt or B) the lecA knockout (ΔlecA) mutant expressing mCherry from pMP7605 were incubated at 37 °C for 24 h with agitation (180 rpm). Biofilms were stained with 17 for 10–30 min. Z‐stacks (232×232 μm) were recorded every 2 μm at 561 nm for mCherry (red) and 488 nm for fluorescein (green). The 3D images show merged images of both channels (488 nm and 561 nm) from top and side views.