Table 3.

Statistics describing the genetic diversity of the Mandenka population based on HLA molecular typings obtained by 3 different techniques, PCR‐SSO, NGS‐454 and NGS‐MiSeq

Locus	N (total)			N (unrelated)			k/ar			H (%)			F50
	PCR‐SSO (second‐field)	NGS‐454	NGS‐MiSeq	PCR‐SSO (second‐field)	NGS‐454	NGS‐MiSeq	PCR‐SSO (second‐field)	NGS‐454	NGS‐MiSeq	PCR‐SSO (second‐field)	NGS‐454	NGS‐MiSeq	PCR‐SSO (seond‐field)	454	NGS‐MiSeq
A	196	–	87	72	–	72	23/21.6	–	22/20.7	92.2	–	92.0	5	–	5
B	198	–	83	67	–	67	30/27.4	–	30/27.8	93.6	–	93.6	6	–	7
C	165	–	83	54	–	54	15/15	–	18/17.9	89.4	–	91.0	4	–	5
DRB1	198	194	81	96	96	65	22/19.0	23/20.3	20/16.9	87.7	87.6	87.6	3	4	4
DQA1	–	194	82	–	66	66	–	9/9	14/13.0	–	60.7	71.5	–	1	1
DQB1	195	196	76	94	96	60	12/10.9	11/10.5	13/12.8	66.2	68.2	76.7	1	1	2
DPA1	–	–	51	–	–	51	–	–	10/10	–	–	71.9	–	–	2
DPB1	193	199	82	99	101	70	18/14.4	14/12.7	19/16.6	80.0	80.7	86.3	2	2	3

N (total), total number of individuals analysed for which the typing yielded usable results; N (unrelated), number of unrelated individuals analysed for which the typing yielded usable results; k, number of alleles detected; ar, allelic richness or number of alleles expected in a population whose size is equal to the smallest N used with a given technique (ie, 54 for PCR‐SSO, 66 for NGS‐454 and 54 for NGS‐MiSeq); H, heterozygosity; F50, number of most frequent alleles whose cumulated frequency reaches at least 50%.

Class I loci were not typed with NGS‐454, DQA1 was not typed with PCR‐SSO, and DPA1 was only typed with NGS‐MiSeq.